Sterilization of allografts is the most important step in ensuring safety and viability of such tissue products. Current sterilization standards such as 25 kGy gamma irradiation can have adverse effects on the ultrastructure and biomechanical properties of allograft tissue. Supercritical CO2 (scCO2) technology, represents an improved sterilization process that is able to preserve tissue properties. scCO2 is not conducted at high temperatures, so it does not denature protein or cause collagen cross linking; this sterilization process preserves the biomechanical properties of the graft.